Translational_Unit

Part:BBa_K734001

Designed by: Erik Quandt   Group: iGEM12_Austin_Texas   (2012-08-08)

Theophylline demethylation pathway

Contains Methylxanthine N-demethylases NdmA, NdmB, NdmC, and associated reductase NdmD from Psuedomonas putida CBB5. Constitutive expression is driven by BBa_J23100 promoter.

Enables demethylation of 1 and 3 methylxanthines such as Theophylline (1,3-methylxanthine).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1229
    Illegal XbaI site found at 1126
    Illegal XbaI site found at 2229
    Illegal XbaI site found at 3109
    Illegal PstI site found at 548
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1229
    Illegal NheI site found at 8
    Illegal NheI site found at 31
    Illegal NheI site found at 1730
    Illegal NheI site found at 1925
    Illegal PstI site found at 548
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1229
    Illegal BglII site found at 2687
    Illegal BglII site found at 3181
    Illegal BglII site found at 3652
    Illegal XhoI site found at 4793
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1229
    Illegal XbaI site found at 1126
    Illegal XbaI site found at 2229
    Illegal XbaI site found at 3109
    Illegal PstI site found at 548
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1229
    Illegal XbaI site found at 1126
    Illegal XbaI site found at 2229
    Illegal XbaI site found at 3109
    Illegal PstI site found at 548
    Illegal AgeI site found at 2224
    Illegal AgeI site found at 3320
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 984
    Illegal BsaI.rc site found at 2221


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